Experiment summary for ENCSR873YHI
doi:10.17989/ENCSR873YHI
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- aptf-3
- Biosample summary
- Caenorhabditis elegans strain OP765 (wgIs765(aptf-3::TY1 EGFP 3xFLAG(p_wTRG5_single_colony_c_0091_5E12);unc-119(+));unc-119 (tm4063)) whole organism young adult (64 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans aptf-3
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing aptf-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:aptf-3_OP765_youngadult_1_IP
- Date released
- November 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP765 (wgIs765(aptf-3::TY1 EGFP 3xFLAG(p_wTRG5_single_colony_c_0091_5E12);unc-119(+));unc-119 (tm4063)) young adult (64 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans aptf-3 | ENCBS139NTQ | ENCGM388NFH | ENCAB728YTO | ENCLB524ANK |
| 2 | 1 | Caenorhabditis elegans strain OP765 (wgIs765(aptf-3::TY1 EGFP 3xFLAG(p_wTRG5_single_colony_c_0091_5E12);unc-119(+));unc-119 (tm4063)) young adult (64 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans aptf-3 | ENCBS557XKQ | ENCGM388NFH | ENCAB728YTO | ENCLB267PKC |