Experiment summary for ENCSR936TED
doi:10.17989/ENCSR936TED
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Target
- POLR2G
- Biosample summary
- Homo sapiens K562 expressing RNAi targeting H. sapiens POLR2G
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with an shRNA knockdown against POLR2G. (K-POLR2G-30B)
- Nucleic acid type
- RNA
- RNA integrity number
- 9.6 [2-1]9.7 [1-1]
- Depleted in
- rRNA
- Size range
- >200
- Extraction method
- Trizol
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- References
- Aliases
- brenton-graveley:K-POLR2G-30B
- Date submitted
- May 14, 2014
- Date released
- June 30, 2014
- Gene Silencing Series
- ENCSR100JYY
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi targeting H. sapiens POLR2G | ENCBS023YVH | ENCGM906MYI | ENCLB477GKN |
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi targeting H. sapiens POLR2G | ENCBS865CZC | ENCGM906MYI | ENCLB575IMJ |