Experiment summary for ENCSR968HCJ
doi:10.17989/ENCSR968HCJ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- unc-130
- Biosample summary
- Caenorhabditis elegans strain AGK541 ((armSi1[Pmex5::UNC-130::GFP::tbb-2 3'UTR; cb-unc-119(+)II]; unc-119(ed3)III; niDf199IV)) whole organism hermaphrodite L4 larva 48 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans unc-130
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing UNC-130-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:UNC-130-AGK541-L4
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF908KBY, ENCFF917GYZ, ENCFF779AJV, ENCFF041RBE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain AGK541 ((armSi1[Pmex5::UNC-130::GFP::tbb-2 3'UTR; cb-unc-119(+)II]; unc-119(ed3)III; niDf199IV)) hermaphrodite L4 larva 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-130 | ENCBS027TPF | ENCGM551JJI | ENCAB728YTO | ENCLB395CZL |
| 2 | 1 | Caenorhabditis elegans strain AGK541 ((armSi1[Pmex5::UNC-130::GFP::tbb-2 3'UTR; cb-unc-119(+)II]; unc-119(ed3)III; niDf199IV)) hermaphrodite L4 larva 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-130 | ENCBS717YPJ | ENCGM551JJI | ENCAB728YTO | ENCLB257BNV |