Experiment summary for ENCSR976OLR
doi:10.17989/ENCSR976OLR
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- hlh-26
- Biosample summary
- Caenorhabditis elegans strain RW12174 (st12174[hlh-26::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) whole organism young adult (66 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using CRISPR targeting C. elegans hlh-26
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing hlh-26-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:hlh-26_RW12174_youngadult_1_IP
- Date released
- November 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain RW12174 (st12174[hlh-26::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) young adult (66 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans hlh-26 | ENCBS508BNZ | ENCGM890KSL | ENCAB728YTO | ENCLB356GHA |
| 2 | 1 | Caenorhabditis elegans strain RW12174 (st12174[hlh-26::GFP+ loxP unc-119(+) + loxP]; unc-119(tm4063) III) young adult (66 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans hlh-26 | ENCBS537TEK | ENCGM890KSL | ENCAB728YTO | ENCLB458PBR |