Functional Characterization Experiment summary for ENCSR259LFO
doi:10.17989/ENCSR259LFO
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transient transfection for SORT1 locus
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- References
- Aliases
- nadav-ahituv:SORT1-1-mpra-satMut-RNA
- Date released
- October 11, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS316VVP | ENCGM402DPZ | ENCLB877JUQ |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS409LQX | ENCGM402DPZ | ENCLB148XBH |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS313OWA | ENCGM402DPZ | ENCLB698LRS |