Functional Characterization Experiment summary for ENCSR515OUJ
doi:10.17989/ENCSR515OUJ
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens NCI-H460 genetically modified (insertion) using transduction, using CRISPRi (pgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- full tiling
- Replication type
- isogenic
- Description
- Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- Treatments
- 2 μg/mL Puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
- Nucleic acid type
- RNA
- Size range
- 710-720
- Strand specificity
- unstranded
- Platform
- Illumina HiSeq 4000
- Controls
- Elements references
- ENCSR327RRR (MYC, MYB)
Attribution
ENCODE4 project
- Lab
- Bing Ren, UCSD
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- bing-ren:BC19E_D14_H460
- Date submitted
- January 31, 2020
- Date released
- August 6, 2020
- Functional Characterization Series
- ENCSR401HFH
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens NCI-H460 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days | ENCBS175XJF | ENCGM554RTS, ENCGM353IMQ | ENCLB444KQB |
| 1 | 1 | Homo sapiens NCI-H460 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days | ENCBS933GQA | ENCGM554RTS, ENCGM353IMQ | ENCLB569QVT |