Functional Characterization Experiment summary for ENCSR552KGE
doi:10.17989/ENCSR552KGE
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Mus musculus F123 genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Dppa2, (insertion) using transduction, using CRISPR cutting (sgRNA) for Dppa2 locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- peak tiling
- Examined loci
- Dppa2 (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina HiSeq 4000
- Controls
- Elements references
- Elements cloning
- ENCSR147EBW
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Low mCherry sorted assay_Dppa2
- Date submitted
- July 30, 2020
- Date released
- August 7, 2020
- Functional Characterization Series
- ENCSR709VRX
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus F123 cell line genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Dppa2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS572NGF | ENCGM802UGA, ENCGM896QXR, ENCGM364WLO | ENCLB821KVZ |
| 2 | 1 | Mus musculus F123 cell line genetically modified (insertion) using CRISPR (sgRNA) targeting M. musculus Dppa2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS411FGL | ENCGM802UGA, ENCGM896QXR, ENCGM364WLO | ENCLB133WLH |