ENCGM002ZNT
Summary
- Status
- released
- Description
- The CETCh-seq method (Savic et al., 2015) was used to edit the genome of a pool of cells, inserting a 3xFLAG epitope at the 3' end of the target gene. The protein contains a C-terminal 3xFLAG tag.
- Type
- insertion
- Tags
- 3xFLAG — C-terminal
- Purpose
- tagging
Modification site
- Target
- ATF1-human
Modification method
- Method
- CRISPR
- Guide RNAs
- TAGGAATCAAACACTTTTATtgg
- Reagents
Documents
PCR analysis Characterization
Caption excerpt: DNA was extracted from 1 million HepG2_FLAG tagged cells for each replicate…
- Lab
- Richard Myers, HAIB
PCR analysis Characterization
Caption excerpt: DNA was extracted from 1 million K562_FLAG tagged cells for each replicate and…
- Lab
- Richard Myers, HAIB