Summary
- Description
- The transgenic strain expressing UNC-130::GFP under mex-5 promoter was generated by Mos1-mediated single copy insertion (MosSCI) transformation (Frokjaer-Jensen et al., 2008) into EG4322 (ttTi5605 II; unc-119(ed3)III) resulting in a strain AGK541 (armSi1[Pmex5::UNC-130::GFP::tbb-2 3'UTR; cb-unc-119(+)II; unc-119(ed3)III). The Pmex5::UNC-130::GFP::tbb-2 3'UTR plasmid was generated by MultiSite Gateway cloning system (Invitrogen) using the plasmids described in (Zeiser et al., 2011). Strain published in Cecere et al., 2012. PMID: 22819322. Received from Jun Liu
- Type
- insertion
- Purpose
- tagging
Modification method
- Nucleic acid delivery method
- bombardment